Hello Dong Li,
for a region different from H-alpha you have to make your own dispersion equation with the Profile - Dispersion window. For that you have to enter the wavelength and pixel position of at least two (better a few more) known calibration lines (Ne Ar etc), which you have to identify first.
For details look here:
http://www.astrosurf.com/buil/isis/tuto ... ion_us.htm
For scaling you choose a region of the continuum where there are no absorption or emission lines. By normalizing the spectrum is multiplied by a factor so that the average in the normalizing region is equal to one. The difference in the scaling in the settings and the normalize button is that the value in the settings is used when a spectrum is reduced from a CCD image, the normalize button can be applied to any spectrum for rescaling later on.
For the response function you can use the spectra from the MILES database in ISIS, which are of lower resolution but should work or you can download spectra from the UVES database
http://www.eso.org/sci/observing/tools/ ... tonow.html
This however is quite difficult because the data cannot be read directly with ISIS, so I would stay with the MILES database for a start.
Load a file from the database and save it as e.g. HDxxxxref.fit for further use.
Hope this helps and clear skies,
Martin